Citizen Science Program Presents
Networks of Exchanging Antibiotic Resistomes in Human and Environmental Microbiota
Wednesday, January 14, 2015
Fisher Center, Sosnoff Theater
Presented by Gautam Dantas
While the most acute effects of increasing antibiotic resistance in pathogens are observed in clinical settings, it is becoming increasingly clear that the evolution and transmission dynamics of resistance gene dissemination is an ecological problem. Indeed, steady use and abuse of antibiotics over the past century in food animals, humans, and the environment has provided substantial selective pressure for enrichment of resistance genotypes in each of their associated microbiomes. An over-reliance on culture-based methods, the standard in the study of clinical resistance, has vastly underestimated these reservoirs of resistance genes (or ‘resistomes’). To address this issue, we have recently developed high-throughput metagenomic functional selections, aided by next-generation sequencing, to characterize resistomes encoded by the microbiota of healthy human adults and children as well as diverse soils. Hundreds of resistance genes we identify from specific taxa in these different microbial communities are identical to resistance genes found in major human pathogens, indicating recent genetic exchange between these microbes. We also find thousands of functionally validated resistance genes which are genetically novel, but flanked by genes involved in horizontal gene transfer, including transposases and integrases. Together, these findings highlight the substantial antibiotic resistome encoded by microbes from diverse environments, which is available for exchange with pathogens, with the potential to severely exacerbate the problems with clinical resistance.Dr. Dantas is an Assistant Professor at the Center for Genome Sciences and Systems Biology at the Washington University School of Medicine in St. Louis, Missouri.
For more information, call 845-758-7490, or e-mail [email protected].
Location: Fisher Center, Sosnoff Theater